Demo cohort 1.fcs
Sample gating strategy
Cross-group report
Cross-group report · Control / Treated · 3 samples
3 samples · 2026-10-03
FlowApp
Report overview
Samples3
Events540,000
Populations2
QC reviewed0
Group summaries
| Group | Population | Valid / included samples (%Parent) | Mean ± SD (%Parent) | Median / range (%Parent) | Mean %Total | Mean MFI | Mean sample median |
|---|---|---|---|---|---|---|---|
| Control | Illustrative cells gate | 1/1 | 83.18% | 83.18% / 83.18%–83.18% | 83.18% (1/1) | - (0/1) | - (0/1) |
| Control | Illustrative CD3+ gate | 1/1 | 80.14% | 80.14% / 80.14%–80.14% | 66.66% (1/1) | - (0/1) | - (0/1) |
| Treated | Illustrative cells gate | 2/2 | 83.15% ± 0.04% | 83.15% / 83.12%–83.18% | 83.15% (2/2) | - (0/2) | - (0/2) |
| Treated | Illustrative CD3+ gate | 2/2 | 80.32% ± 0.16% | 80.32% / 80.2%–80.43% | 66.79% (2/2) | - (0/2) | - (0/2) |
Included samples
| Sample | Group | Events | Gated populations with statistics | QC |
|---|---|---|---|---|
| Demo cohort 1.fcs | Control | 180,000 | 2/2 | QC not run |
| Demo cohort 2.fcs | Treated | 180,000 | 2/2 | QC not run |
| Demo cohort 3.fcs | Treated | 180,000 | 2/2 | QC not run |
Control · Group plots
Samples plotted: 1/1 · Population events: 180,000
Samples plotted: 1/1 · Population events: 149,722
Samples plotted: 1/1 · Population events: 119,980
Treated · Group plots
Samples plotted: 2/2 · Population events: 360,000
Samples plotted: 2/2 · Population events: 299,356
Samples plotted: 2/2 · Population events: 240,428
Custom analysis
Descriptive only · Correction：Benjamini–Hochberg FDR · α=0.05
Group by：Selected report groups · Experimental-unit ID：Not confirmed
Individual dots · 1. Control · 2. Treated
Mean and error bars · SD · 1. Control · 2. Treated
| Population | Control | Treated |
|---|---|---|
| Illustrative cells gate | 83.179n=1 | 83.154n=2 |
| Illustrative CD3+ gate | 80.135n=1 | 80.315n=2 |
Colors and values represent sample means per group; missing values remain empty.
Descriptive statistics
| Population | Group | Metric | Valid / included | Mean | Median | SD | SEM | Minimum – maximum |
|---|---|---|---|---|---|---|---|---|
| Illustrative cells gate | Control | %Parent | 1/1 | 83.179 | 83.179 | — | — | 83.179 – 83.179 |
| Illustrative cells gate | Treated | %Parent | 2/2 | 83.154 | 83.154 | 0.042 | — | 83.124 – 83.184 |
| Illustrative CD3+ gate | Control | %Parent | 1/1 | 80.135 | 80.135 | — | — | 80.135 – 80.135 |
| Illustrative CD3+ gate | Treated | %Parent | 2/2 | 80.315 | 80.315 | 0.162 | — | 80.201 – 80.43 |
Each point represents a sample measurement, not an event. Missing measurements are not zero. Box whiskers use 1.5 × IQR; with fewer than five measurements, only dots are shown. Violin densities are estimates and require at least five nonconstant observations. Confidence intervals are pointwise, not multiplicity-adjusted. Corrections cover all valid tests across metrics and populations in this custom analysis. Omnibus tests do not identify which groups differ; no post-hoc comparisons are implied.
Sample count
3
Files included in this report
Group count
2
Selected groups
Acquired events
540,000
Sum of acquired events; not replicate n
Samples with QC results
0
No QC conclusion for unreviewed samples
Data overview
Data overview
-
1
The report includes 3 samples; group summaries retain individual sample measurements.
-
2
QC results are available for 0 samples.
-
3
Event counts describe acquired cells, not independent biological replicates.
Demo cohort 2.fcs
Sample gating strategy
Demo cohort 3.fcs
Sample gating strategy
Demo cohort 1.fcs
Sample population plots
Demo cohort 2.fcs
Sample population plots
Demo cohort 3.fcs
Sample population plots
Demo cohort 1.fcs
Sample population statistics
| Population | Gating axes | Events | %Parent | %Total | MFI | Median |
|---|---|---|---|---|---|---|
| Illustrative cells gate | FSC-A / SSC-A | 149,722 | 83.18% | 83.18% | - | - |
| Illustrative CD3+ gate | CD3 FITC / SSC-A | 119,980 | 80.14% | 66.66% | - | - |
Demo cohort 2.fcs
Sample population statistics
| Population | Gating axes | Events | %Parent | %Total | MFI | Median |
|---|---|---|---|---|---|---|
| Illustrative cells gate | FSC-A / SSC-A | 149,732 | 83.18% | 83.18% | - | - |
| Illustrative CD3+ gate | CD3 FITC / SSC-A | 120,086 | 80.2% | 66.71% | - | - |
Demo cohort 3.fcs
Sample population statistics
| Population | Gating axes | Events | %Parent | %Total | MFI | Median |
|---|---|---|---|---|---|---|
| Illustrative cells gate | FSC-A / SSC-A | 149,624 | 83.12% | 83.12% | - | - |
| Illustrative CD3+ gate | CD3 FITC / SSC-A | 120,342 | 80.43% | 66.86% | - | - |
Demo cohort 1.fcs
Sample quality control
QC not run.
Demo cohort 2.fcs
Sample quality control
QC not run.
Demo cohort 3.fcs
Sample quality control
QC not run.
Metadata and workflow audit
Metadata
- Demo cohort 1.fcsgroup: - · condition: Control · timepoint: - · replicate: -
- Demo cohort 2.fcsgroup: - · condition: Treated · timepoint: - · replicate: -
- Demo cohort 3.fcsgroup: - · condition: Treated · timepoint: - · replicate: -
Recent workflow steps
- No workflow steps recorded.
Chart and statistics notes
- %Parent is the percentage of the parent population; %Total is the percentage of all sample events. MFI and median use the specified statistics channel. Missing measurements are not zero.
- Group summaries give equal weight to each sample, rather than pooling events. Counts show valid / included samples for each metric; file counts are not necessarily biological replicate counts.
- Group cytometry plots pool available events for visualization only; they do not replace sample-level statistics or biological replicates.
- Gating plots for the named sample. Gate percentages refer to the immediate parent population.
- Sample-specific population distributions: CD3, CD4, CD8, LIVE/DEAD, CD19, CD45. Event counts are not biological replicate n.