CytoDesk / FlowApp
Flow cytometry resources
Work through an analysis task, check a statistic, or explore an example report. These resources connect FCS data, population definitions and reviewable results.
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Start an analysis
- TutorialOpen and inspect an FCS file
Check event counts, channels and the plot before defining populations.
- Tutorial and worked exampleFrom gated samples to an editable report
Choose report objects, charts and statistics, then arrange and annotate the output.
Gating and statistics
- Explanation and calculator%Parent, %Total and fluorescence summaries
Change event counts to see how the same child population has two different percentages.
- Analysis conceptsParent, child, quadrant and Boolean gates
Understand the event set behind a gate, not just the boundary drawn on a plot.
Prepare data
- Tutorial and troubleshootingRun QC, review marks and troubleshoot a batch
Read method-specific results, distinguish marks from event removal and reproduce a synthetic QC example.
- TroubleshootingNo compensation matrix detected: what next?
Distinguish an embedded matrix, control-based calculation and an unresolved data limitation.
- TutorialUse metadata to organize sample groups
Keep source information, add experimental labels and create groups explicitly.
Examples and validation
- Interactive synthetic dataOpen the FlowApp demo cohort
Explore three synthetic immune-panel files without preparing your own data.
- Complete synthetic reportOpen an editable group-report example
Inspect the actual output, change layout and annotations, and save a revised HTML. No biological conclusions.

- Published validation caseCell-cycle public-data validation
Review the stated checks and limitations. This is separate from the synthetic software demo.
- Product questionsReports, local history, QC and optional AI
Check what is saved, what requires a model provider and which results need researcher review.
Guides describe FlowApp behavior and general analysis concepts; they do not replace assay-specific controls or a validated laboratory procedure. Synthetic examples are labeled separately from experimental evidence.
FlowApp team · Updated .